The dataset consists of human cells from the bone marrow and the CD34+ Enriched Bone Marrow Cells.
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Data was preprocessed using the canonical Seurat protocol.
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Analysis of Batch Effect and Integration.
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Dimensionality Reduction and Clustering using PCA and UMAP.
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Both Manual (CellMarker) and Automatic Cell Type Annnotation (SingleR) Performed.
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Trajectory Analysis (Monocle3).
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Differential Expression Analysis (Seurat).
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Pathway Analysis (EnrichR).
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Cell-Cell Communication (CellChat)