@@ -36,11 +36,11 @@ struct PredefinedStruct {
3636// predefined settings for known barcode/search schemes
3737// new setting added to this map will automatically be available
3838static const map< string, PredefinedStruct> predefinedMap = {
39- {" 10x3v3" , {" 10x version 3 chemistry 3' " , // option string and description for help information
39+ {" 10x3v3" , {" 10x version 3 3' chemistry " , // option string and description for help information
4040 " -x CTACACGACGCTCTTCCGATCT -b ???????????????? -u ???????????? -x TTTTTTTTT -f 8 -e 2" }}, // settings
41- {" 10x3v2" ,{" 10x version 2 chemistry 3' " ,
41+ {" 10x3v2" ,{" 10x version 2 3' chemistry " ,
4242 " -x CTACACGACGCTCTTCCGATCT -b ???????????????? -u ?????????? -x TTTTTTTTT -f 8 -e 2" }},
43- {" 10x5v2" ,{" 10x version 2 chemistry 5' " ,
43+ {" 10x5v2" ,{" 10x version 2 5' chemistry " ,
4444 " -x CTACACGACGCTCTTCCGATCT -b ???????????????? -u ?????????? -x TTTCTTATATGGG -f 8 -e 2" }},
4545 {" 10x_atac" ,{" 10x ATAC-Seq" ,
4646 " -x ACCGAGATCTACAC -b ???????????????? -x CGCGTCTGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG -f 8 -e 2" }},
@@ -57,11 +57,11 @@ void print_usage(){
5757
5858 cerr << " options: \n " ;
5959 cerr << " -k known_list Either 1) a text file of expected barcodes in the first column,\n " ;
60- cerr << " one row per barcode, or 2) a comma separate string of barcodes.\n " ;
60+ cerr << " one row per barcode, or 2) a comma-separated string of barcodes.\n " ;
6161 cerr << " Without this option, flexiplex will search and report possible barcodes.\n " ;
6262 cerr << " The generated list can be used for known_list in subsequent runs.\n " ;
6363 cerr << " -l true/false Replace read ID with barcodes+UMI (default: true)\n " ;
64- cerr << " -r true/false Remove search strings including flanking sequenence and split read\n " ;
64+ cerr << " -r true/false Remove search strings including flanking sequence and split read\n " ;
6565 cerr << " if multiple barcodes found (default: true).\n " ;
6666 cerr << " -s true/false Sort reads into separate files by barcode (default: false)\n " ;
6767 cerr << " -c true/false Add a _C suffix to the read identifier of any chimeric reads\n " ;
@@ -74,7 +74,7 @@ void print_usage(){
7474 cerr << " -f N Maximum edit distance to primer+polyT (default 8).\n " ;
7575 cerr << " -p N Number of threads (default: 1).\n\n " ;
7676
77- cerr << " Specifying adaptor / barcode structure : \n " ;
77+ cerr << " Specifying adaptor / barcode structure: \n " ;
7878 cerr << " -x sequence Append flanking sequence to search for\n " ;
7979 cerr << " -b sequence Append the barcode pattern to search for\n " ;
8080 cerr << " -u sequence Append the UMI pattern to search for\n " ;
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