PTATO for ResolveOME v2 data

[tobyaicher]

Hi,

I wanted to ask if you have any experience running PTATO on ResolveOME v2 DNA data (using the commericial bioskryb kit).

I would think the majority of errors in ResolveOME DNA data are similar to those you would see with PTA alone. But there would be additional errors due to RNA contamination / RNA editing that a model trained on PTA data alone would not capture.

Have you trained other models for ResolveOME data or have advice on settings/parameters that could be changed to help running PTATO on ResolveOME v2 DNA data.

Thank you fantastic publication and this looks like a great tool.

TA

[mroosmalen]

Hi,

We are currently busy with analyzing ResolveOME V2 DNA data. We see that we need to add an additional filtering step, because there are some new additional artifacts with this kit.

Best
Mark

[moshl]

Hi,

We are currently busy with analyzing ResolveOME V2 DNA data. We see that we need to add an additional filtering step, because there are some new additional artifacts with this kit.

Best Mark

Hi Mark，Can the new filtering steps be updated to PTATO? The fact that 9,000 mutations in scWES data （produced by PTATO） ended up passing is very strange.

Best Mo