Unable to find delta cq values for qPCRBatch. example from the example data

[GK0745]

Hi,
The script used
qPCRBatch.example <- read.qPCR(qPCR.example)
hkgs<-"Actb-Rn00667869_m1"
qPCRBatch.norm <- deltaCq(qPCRBatch = qPCRBatch.example, hkgs = hkgs, calc="arith")

Error` in .local(qPCRBatch, ...) :
given housekeeping gene, Actb.Rn00667869_m1 not found in file. Ensure entered housekeeping genes appear in the file

Can you please help me in understanding this.

Thank you
Gyanendra

[stamats]

Dear Gyanendra,

there is not gene with this name in the dataset; compare
featureNames(qPCRBatch.example)

Best regards,
Matthias

[GK0745]

Dear Matthias,
Thank you for the help, I have another basic question about the example data. The qPCRBatch.taqman detector column has m_1, _s1, and _g1 and they are accompanied with Rn values which vary across the genes. Can you please help in understanding the varied Rn values of each gene.

Good Day
Gyanendra

[stamats]

Dear Gyanendra,

I'm not familiar with taqman data. Hence, hopefully James will read your comment and answer.

Best regards,
Matthias