For images with many cells in the field of view, it might be tricky to use a region with no cells for the automatic estimation of the prior, even if the ROI of the cell of interest is present.
If multiple frames are available as reference (ideally with no cells), one could use them for this purpose even if no ROI was provided.
For images with many cells in the field of view, it might be tricky to use a region with no cells for the automatic estimation of the prior, even if the ROI of the cell of interest is present.
If multiple frames are available as reference (ideally with no cells), one could use them for this purpose even if no ROI was provided.