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FUCCI Track and Spot Analysis

This repository contains Jupyter notebooks for analyzing and visualizing tracking, spot detection, and nuclei segmentation data from the Kurppa dataset.


Deep learning pipeline

Nuclei_detection_pipeline.ipynb

Open In Colab

Implements a deep learning-based pipeline for nuclei detection:

  • Loads raw microscopy images
  • Applies pretrained segmentation model
  • Outputs masks and postprocessed features
  • Can be integrated with tracking and spot analysis workflows

Analysis Notebooks

1_Tracks_analysis+plotting.ipynb

Open In Colab

Performs analysis of track data, including:

  • Filtering and preprocessing of trajectories
  • Calculating metrics like speed, displacement, and direction
  • Summary statistics and initial visualizations

2_Spots_analysis.ipynb

Open In Colab

Analyzes spot detection data:

  • Aggregates spot-level features
  • Calculates statistical descriptors
  • Compares spot distributions across experimental conditions

3_Spots_plotting.ipynb

Open In Colab

Generates visualizations for spot analysis results:

  • Box plots, histograms, and scatter plots
  • Supports grouped comparisons
  • Prepares publication-ready figures

How to Use

To run the notebooks in Google Colab, click on the "Open in Colab" badges above.
Make sure to upload your dataset or mount your Google Drive as needed.


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FUCCI analysis pipeline

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