Dataset of an ELISA test using recombinant B8/2 subunit (IgG rAg8/2) for Echinococcus granulosus sensu lato.
This supplements the data in brief manuscript by T. V. Poggio et al., "Immunodiagnosis of cystic echinococcosis in livestock: Development and validation dataset of an ELISA test using a recombinant B8/2 subunit of Echinococcus granulosus sensu lato", 2022, https://pubmed.ncbi.nlm.nih.gov/35669005/
Description of the Files:
DataFig2.csv contains the IgG Optical Density data of the following:
Negative (Serum) Panel: Sera collected from healthy Merino lambs aged under 3 months, belonging to the non-endemic area of Puerto Madryn (Chubut Province, Argentina) (n=79).
Positive (Serum) Panel: Adult sheep from the Chubut Province, each experimentally infected per os with 2,000 E. granulosus s.l. eggs in May 2013, necropsied two years later in 2015. Live cysts (from 2 to 249) larger than 10 mm were detected in all animals, demonstrating E. granulosus s.l. infection (n=36).
Other Parasites (Cross-Reactive Sera): Sera from sheep naturally infected with Taenia hydatigena, Thysanosoma actinioides and Fasciola hepatica were collected from Río Negro Province (n=20).
(Laboratory) Positive control: control positive read in each plate.
(Laboratory) Negative control: control negative read in each plate.
(Laboratory) Control without Ag: control no antigen read in each plate.
DataFig3.csv contains the IgG Optical Density data on the Humoral Response Kinetics experiment.
In this experiment, six-month old female sheep (n=5) were each experimentally infected per os with 2,000 E. granulosus s.l. eggs. Sera were collected by obtaining blood samples every 7 to 15 days for 49 weeks for investigation of the temporal kinetics of IgG antibody response to the rAg8/2 antigen measured as optical density (OD) at 405nm.
Headings in the file are as follows:
Week: Experimental week number post infection.
Date: Date where the sample was taken.
Sheep 117, 130, 131, 133, 134: IgG Optical Density for each of those five animals during the experiment.
Fig2 is the ilustration (boxplot) generated from DataFig2.csv
Figure Caption: Box plots of IgG Optical Density (OD) 405nm from sheep sera: Negative Serum Panel (n=79), lambs under 3 months of age from a CE-free area; Positive Serum Panel (n=36), adults experimentally infected with positive necropsy; Cross-reactive Sera (n=20), animals with other parasites. The solid black horizontal line within the boxes is the median; the lower and upper borders are, respectively, the 1st (Q1) and 3rd (Q3) quartiles; the vertical bars indicate the ‘minimum’ and ‘maximum’ values, calculated as Q1–1.5 × IQR (interquartile range) and Q1+1.5 × IQR, respectively. The circles represent the outliers. Laboratory (positive, negative and no-antigen) controls are presented to the right of the vertical dotted line.
Fig3 is the ilustration (plot) generated from DataFig3.csv
Figure Caption: Average Optical Density (OD)405nm of antibody levels against rAg8/2 in serum samples from sheep experimentally infected with 2,000 E. granulosus s.l. eggs (n=5) plotted against time post infection (in weeks) over 49 weeks. The error bars are the standard errors of the means (SEM).
ScriptFigures.R contains the code to generate the figures.