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DLBCL-STORM Hybrid Capture Panel

Files associated with the DLBCL-STORM (Somatic Tracking Of Residual Mutations) hybrid capture panel. The panel was created to detect mutations in cfDNA from DLBCL patients and track those variants accross mutliple timepoints to assess response to treatment.

The full panel is over 618 kb of total target space covering the coding space of 195 genes and 61 non-coding targets subject to aSHM.

For an example bioinformatics pipeline used to analyse data captured by this panel see: https://github.com/LCR-BCCRC/lcr-modules/tree/cfdna_pipeline/modules/cfdna_pipeline/1.0

Bed files

File Description
DLBCL_STORM_FullPanel_Jan2025.bed Full panel with coding and non-coding targets
DLBCL_STORM_aSHM_Jan2025.bed aSHM (non-coding) targets
DLBCL_STORM_exons_Jan2025.bed Coding targets

background mutation rates

File Description
*STORM_background_mutation_rates_JAN2026.tsv Index of genomic positions and the point and indel mutation rates and SD generated using the lcr-module artifact_alert, an a panel of over 300 buffy coat samples from DLBCL patients

Hotspots

File Description
mutation_Hotspots_hg38_June2025.txt Custom DLBCL hotspot manifest based on data from the GAMBL project
lymphopedia_DLBCL_hotspots_hg38_2026-01-02.vcf.gz Hotspot manifest generated using samples from the GAMBL repository (reference in prep)

GAMBL: https://github.com/morinlab/GAMBLR

Notspots

File Description
mutation_Notspots_hg38_June2025.txt Custom DLBCL notspot manifest (aka blacklist) based on data from the GAMBL project
technical_artifact_notspots.vcf A .vcf of positions that were commonly mutated in a large panel of normals sequenced using DLBCL-STORM.

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