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HPC-Organoid-Quant is a modular image analysis pipeline for quantifying organoids in brightfield microscopy images, with optional GFP analysis. It handles fragmented structures, extracts key features, and outputs visual overlays and CSV reports. Runs in Google Colab or locally via Conda or Python venv.
Code and workflows for the study 'Hepatic stellate cell single-cell atlas reveals a highly similar activation process across liver disease aetiologies'—highlighting conserved HSC activation states and biomarkers like COLEC10.